کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2594640 1132275 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Neonatal administration of diethylstilbestrol has adverse effects on somatic cells rather than germ cells
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Neonatal administration of diethylstilbestrol has adverse effects on somatic cells rather than germ cells
چکیده انگلیسی

Neonatal administration of diethylstilbestrol (DES) to rodents has adverse effects on spermatogenesis. However, not many studies have been conducted to determine which type of cell – germ or somatic – is the major target of DES. In order to clarify this, we tried reciprocal germ cell transplantation—transplantation of germ cells from DES-treated mice into intact mice and germ cells from normal mice into DES-treated mice. The donor germ cells were tagged with the green fluorescent protein (GFP) gene in order to distinguish the exogenous germ cells from the endogenous cells. Moreover, to obtain a large number of spermatogonia from the testes of adult mice, we performed fractionation by centrifugation with Percoll. Consequently, we found that the germ cells collected from DES-treated mice have differentiated into normal sperms in normal seminiferous tubules. However, in the case of the transplantation of normal germ cells into the seminiferous tubules of DES-treated mice, defective spermatogenesis was observed. In conclusion, DES has adverse effects on the somatic cells that are involved in spermatogenesis rather than the germ cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reproductive Toxicology - Volume 22, Issue 4, November 2006, Pages 746–753
نویسندگان
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