کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2595056 1132287 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fluconazole alters CYP26 gene expression in mouse embryos
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Fluconazole alters CYP26 gene expression in mouse embryos
چکیده انگلیسی
Disruption of embryonal retinoic acid homeostasis has been postulated to represent an etiological factor involved in the onset of fluconazole-induced teratogenesis. In the present study the impact of a teratogenic pulse of fluconazole on the gene expression of cytochrome P450 (CYP) 26 isoforms, which plays a central role in maintaining proper retinoic acid levels by mediating its degradation, was investigated. ICR pregnant mice were orally administered with 0 (vehicle) or 700 mg/kg of fluconazole on gestation day 8. Embryos were collected 12, 24 and 48 h after treatment. Quantitative real-time reverse-transcription polymerase chain reaction (quantitative real-time RT-PCR) assay was used to quantify the mRNA expression of CYP26a1, CYP26b1 and CYP26c1 in embryos. As result, fluconazole exposure was associated to an up-regulation of CYP26a1, CYP26b1, whereas no significant change was identified for the CYP26c1 isoform. This study demonstrates the capacity of fluconazole to alter CYP26 gene expression in mouse embryos.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reproductive Toxicology - Volume 27, Issue 2, April 2009, Pages 199-202
نویسندگان
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