کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2595165 1132296 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Spermatozoa nucleus takes up lead during the epididymal maturation altering chromatin condensation
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Spermatozoa nucleus takes up lead during the epididymal maturation altering chromatin condensation
چکیده انگلیسی

Lead (Pb) alters sperm chromatin condensation (CC) and the mechanisms are investigated. During spermatogenesis, protamines replace histones and disulfide bonds formation during epididymal maturation condense the chromatin. We evaluated sperm Pb uptake in testis and epididymis and the effects on CC in mice (0.06% Pb2+/16 weeks/drinking water). Spermatozoa from caput epididymis (CP) and cauda epididymis–vas deferens (CE–VD) were obtained and CC was measured by SCSA. Lead levels in spermatozoa from CP were lower than those from CE–VD, and correlated with a decreased CC, while Pb in CE–VD spermatozoa correlated with an increased CC. Lead accumulation into the nucleus was observed and Pb binding to nuclear sulfhydryl groups decreased chromatin decondensation in vitro. Our results suggest that spermatozoa take up Pb during testicular development and epididymal transport and alter CC, depending of the timing of Pb incorporation into the sperm nucleus, which finally may interfere with the chromatin decondensation process after fertilization.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reproductive Toxicology - Volume 21, Issue 2, February 2006, Pages 171–178
نویسندگان
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