کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2595507 1562330 2014 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparative in vivo and in vitro analysis of possible estrogenic effects of perfluorooctanoic acid
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Comparative in vivo and in vitro analysis of possible estrogenic effects of perfluorooctanoic acid
چکیده انگلیسی

Previous studies suggested that perfluorooctanoate (PFOA) could activate the estrogen receptor (ER). The present study examined the hypothesis that PFOA can activate ER using an in vivo uterotrophic assay in CD-1 mice and an in vitro reporter assay. Pre-pubertal female CD-1 mice fed an estrogen-free diet from postnatal day (PND)14 through weaning on PND18 were administered 0, 0.005, 0.01, 0.02, 0.05, 0.1, or 1 mg/kg PFOA or 17β-estradiol (E2, 0.5 mg/kg) from PND18–20. In contrast to E2, PFOA caused no changes in the relative uterine weight, the expression of ER target genes, or the morphology of the uterus/cervix and/or vagina on PND21. Treatment of a stable human cell line containing an ER-dependent luciferase reporter construct with a broad concentration range of PFOA caused no change in ER-dependent luciferase activity; whereas E2 caused a marked increase of ER-dependent luciferase activity. These data indicate that PFOA does not activate mouse or human ER.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology - Volume 326, 4 December 2014, Pages 62–73
نویسندگان
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