کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2598492 | 1562623 | 2016 | 7 صفحه PDF | دانلود رایگان |
• We analysed blood samples of 181 male and 61 female volunteers with a mobile field kit.
• Reference values were calculated for acetyl- and butyrylcholinesterase.
• The large inter-individual variability of cholinesterase values calls for determination of individual pre-exposure values.
• The mobile field kit, ChE check mobile, proved to be a suitable tool for on-site analysis of cholinesterase activities.
Poisoning by organophosphorus compounds (OP) still poses a major medical challenge. Diagnosis of clinical signs of OP poisoning is still the most important parameter for the initiation of specific treatment. However, in case of unspecific signs and of delayed onset of cholinergic crisis a rapid, reliable and on-site analysis of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activity would be of great value. Recently the ChE check mobile, a CE-certified ready to use kit for the determination of whole blood AChE and BChE activities, was developed. Here, we evaluated whole blood AChE and BChE reference values with samples taken from 181 male and 61 female volunteers and analyzed them on-site with the ChE check mobile test kit. The analysis of the data revealed a large inter-individual variability (BChE > AChE), only a small sex difference for AChE but a significant difference for BChE activities. The now available normal range values enable an evaluation of determined AChE and BChE activities in case of suspected exposure to OP nerve agents and pesticides. However, the large inter-individual variability of AChE and BChE activities calls for the determination of pre-exposure values in specific subpopulations in order to enable the diagnosis of low-level OP exposure.
Journal: Toxicology Letters - Volume 249, 13 May 2016, Pages 22–28