کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2598677 1133146 2015 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mutagenic potential of the isoflavone irilone in cultured V79 cells
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Mutagenic potential of the isoflavone irilone in cultured V79 cells
چکیده انگلیسی


• The isoflavone irilone was tested in cultured Chinese hamster lung fibroblast V79, and…
• Inhibited proliferation and did not increase the mutant frequency in the HPRT assay.
• Altered significantly the mutation spectrum.
• Significantly increased micronuclei with chromosomal fragments.
• Disrupted mitosis and significantly increased micronuclei with whole chromosomes.

After consumption of red clover-based dietary supplements, plasma concentrations of the isoflavone irilone (IRI) equal that of the well-investigated daidzein. Since some isoflavones are genotoxic, the potential of IRI to induce mutations was investigated.Gene mutations were determined by hypoxanthine-guanine phosphoribosyltransferase (HPRT) assay and sequencing of mutant cDNA, chromosome and genome mutations by micronucleus assay complemented by immunochemical staining of centromere proteins and microtubules in cultured V79 cells. Cell proliferation was monitored by electronic cell counting, flow cytometry and fluorescence microscopy.IRI did not affect the mutant frequency in the Hprt locus but altered the mutation spectrum by increasing the proportion of deletions and decreasing that of base pair substitutions. Induction of chromosome mutations was supported by a slight but significant increase in the number of micronucleated cells containing chromosomal fragments despite activation of three cell cycle checkpoints possibly interfering with micronuclei formation. Moreover, IRI exhibited a strong aneugenic potential characterized by disrupted mitotic spindles, mitotic arrest, and asymmetrical cell divisions leading to chromosome loss, nuclear fragmentation as well as mitotic catastrophe. Thus, IRI might be another isoflavone to be taken into account in safety assessment of dietary supplements.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Letters - Volume 234, Issue 2, 16 April 2015, Pages 81–91
نویسندگان
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