کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2600049 1133249 2011 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Gene expression profile of terminal end buds in rat mammary glands exposed to diethylstilbestrol in neonatal period
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Gene expression profile of terminal end buds in rat mammary glands exposed to diethylstilbestrol in neonatal period
چکیده انگلیسی

Diethlstilbestrol (DES) is a synthetic estrogen prescribed to several millions of pregnant women worldwide. The risk for breast cancer after age 40 in women prenatally exposed to DES has been reported; however, the precise mechanism of susceptibility to breast cancer remains to be resolved. We investigated the global gene expression profile of terminal end buds (TEBs), the target of carcinogen, in rat mammary glands neonatally exposed to a low- or high-dose DES at postnatal days (PND) 35 and 45, equivalent to the peripubertal stage in humans. In all groups, the number of TEBs gradually increased, peaked at PND35 and decreased at PND49. At PND35 and 49, the low-dose DES-treated group (low-DES group) showed the highest number of TEBs. In the low-DES group at PND35, β-casein, γ-casein and whey acidic protein (WAP) mRNA expression increased 8.2-fold, 26.1-fold and 13.3-fold, respectively, whereas γ-casein and WAP mRNA decreased 17.6-fold and 27.7-fold, respectively, at PND49. The most significant network revealed by Ingenuity Pathway Analysis (IPA) software showed the relevance of NF-κB in low-DES group. The present findings suggest that the deregulation of mammary gland differentiation and development-related genes could be induced and cause the increased number of terminal duct lobular units (TDLUs) in human mammary glands of DES daughters in a critical period of mammary gland development.


► The risk for breast cancer after age 40 in women prenatally exposed to DES has been reported.
► We investigated the global gene expression profile of terminal end buds (TEBs) in rats neonatally exposed to DES.
► Casein expression increase at postnatal days (PND) 35 and decrease at PND 49.
► The most significant network revealed by IPA software was NF-kappa B.
► The deregulation of mammary gland differentiation and development-related genes may be attributable to the increased number of TDLUs in human.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Letters - Volume 205, Issue 1, 10 August 2011, Pages 15–25
نویسندگان
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