Amitriptyline induces coenzyme Q deficiency and oxidative damage in mouse lung and liver

Amitriptyline is a tricyclic antidepressant commonly prescribed for the treatment of several neuropathic and inflammatory illnesses. We have already reported that amitriptyline has cytotoxic effect in human cell cultures, increasing oxidative stress, and decreasing growth rate and mitochondrial activity. Coenzyme Q (CoQ), a component of the respiratory chain and a potent antioxidant, has been proposed as a mitochondrial dysfunction marker. In the present work we evaluated lipid peroxidation, a consequence of oxidative stress, and CoQ level in liver, lung, kidney, brain, heart, skeletal muscle, and serum of mice treated with amitriptyline for two weeks. Lipid peroxidation was increased in a dose-dependent manner in all tissues analyzed. CoQ levels were increased in brain, heart, skeletal muscle, and serum, and strongly decreased in liver and lung. The relation between amitriptyline, CoQ, and oxidative stress is discussed.

► Coenzyme Q (CoQ) has been proposed as a mitochondrial dysfunction marker.
► The antidepressant amitriptyline (Amit) increases oxidative stress, altering mitochondria.
► We show lipid peroxidation in Amit-treated mice and low CoQ level in liver and lung.
► We suggest these organs as the most sensitive to Amit treatment.
► We support the recommendation of CoQ supplementation in patients treated with Amit.