کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2600624 1133276 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Low-concentration tributyltin perturbs inhibitory synaptogenesis and induces neuronal death in immature but not mature neurons
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Low-concentration tributyltin perturbs inhibitory synaptogenesis and induces neuronal death in immature but not mature neurons
چکیده انگلیسی

Tributyltin (TBT) has harmful effects on invertebrates. Reports indicate that intoxication of humans with organotin compounds could be associated with neurological symptoms such as epilepsy and amnesia; however, the toxicity mechanisms in mammals are unknown. TBT acts as a Cl−/OH− antiporter, and likely affects the GABAergic system by disturbing Cl− homeostasis. This study aimed to elucidate neurotoxic actions of TBT on mouse neocortical neurons during development. From 4 days in vitro (4 DIV) or 14 DIV in culture, cortical neurons were exposed to TBT continuously for 3 days. TBT-induced neuronal death at 30 nM during DIV 4–6, and at 50 nM during DIV 14–16. To further characterize this age-dependent cytotoxicity, miniature postsynaptic currents (mPSCs) were analyzed by whole-cell patch-clamp. The frequency of mPSCs was significantly reduced by treatment with 30 nM TBT during DIV 4–6, but not DIV 14–16. After TBT treatment during DIV 4–6, GABAA receptor-mediated reversal potentials (EGABA) were significantly shifted negatively. The TBT-induced EGABA shift and neuronal death were reversed by increment of extracellular Cl− concentration, suggesting that disruption of Cl− homeostasis underlies the disturbance of neuronal ontogeny induced by TBT. These data indicate that the TBT may affect synaptogenesis and neuronal survival, particularly in early development.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Letters - Volume 198, Issue 2, 5 October 2010, Pages 282–288
نویسندگان
, , , ,