کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2600633 1133277 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Pharmacological concentration of resveratrol suppresses aromatase in JEG-3 cells
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Pharmacological concentration of resveratrol suppresses aromatase in JEG-3 cells
چکیده انگلیسی

Estrogen is crucial in preparing of pregnancy, and its role in the maintenance of pregnancy has yet to be elucidated. During the course of pregnancy, the placenta is responsible for the provision of estrogen. The hormone biosynthesis is catalyzed by cytochrome P450 (CYP) 19 or aromatase. In the present study, we screened several common dietary components and identified the grape polyphenol resveratrol to be a potential inhibitor in the hormone synthesis. In a recombinant protein system resveratrol inhibited the aromatase activity with an IC50 value of ∼40 μM. Subsequent analysis was performed in the human placental JEG-3 cells, and 25 μM resveratrol significantly reduced the mRNA abundance in these cells. Since the transcriptional control of CYP19 gene is tissue-specific and the proximal promoter region of exon Ia has previously been shown to be crucial in CYP19 expression in placental cells, we also evaluated the promoter activity of this gene. Reporter gene assays revealed that resveratrol repressed the transcriptional control of promoter Ia. The present study illustrated the possibility that dietary supplementation of resveratrol interfered with the normal functioning of placental cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Letters - Volume 173, Issue 3, 28 September 2007, Pages 175–180
نویسندگان
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