کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2602517 | 1133776 | 2013 | 9 صفحه PDF | دانلود رایگان |
• Transient ERK1/2 and BadSer112 activation by non-toxic 6-OHDA led to cell survival.
• cAMP induced by toxic 6-OHDA stimulated sustained ERK1/2 and BadSer155 activation.
• cAMP–ERK1/2–Bad activation by 6-OHDA modulated on cell viability in PC12 cells.
The roles of cyclic AMP (cAMP)–ERK1/2–Bad signaling pathways in 6-hydroxydopamine (6-OHDA)-induced cell survival and death were investigated. In PC12 cells, 6-OHDA (10–100 μM) concentration-dependently increased the intracellular levels of cAMP mediated by the Ca2+-CaMKII-adenylyl cyclase system. 6-OHDA at the non-toxic level (10 μM) induced transient ERK1/2 phosphorylation and BadSer112 phosphorylation, which maintained cell survival. In contrast, the high levels of cAMP induced by toxic levels (50 and 100 μM) of 6-OHDA induced sustained ERK1/2 phosphorylaton and BadSer155 phosphorylation. The cells then moved to cell death process through Bcl2 phosphorylation and caspase-3 activation. BadSer155 phosphorylation by 6-OHDA was inhibited by PKA (H89) and MEK (U0126) inhibitors, indicating that it was mediated via the cAMP–PKA-sustained ERK1/2 system. In SK-N-BE(2)C cells, the non-toxic level of 6-OHDA also showed transient ERK1/2 phosphorylation and BadSer112 phosphorylation, and toxic levels of 6-OHDA exhibited sustained ERK1/2 phosphorylation and BadSer155 phosphorylation. These results suggest that ERK1/2 phosphorylation by 6-OHDA shows biphasic functions on cell survival and death in PC12 cells. It is, therefore, proposed that the cAMP–ERK1/2–Bad signaling pathways incurred by toxic levels of 6-OHDA play a role in dopamine neuron death of animal models of Parkinson’s disease.
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Journal: Toxicology in Vitro - Volume 27, Issue 8, December 2013, Pages 2233–2241