کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2602984 | 1133803 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Butyrate reduces the frequency of micronuclei in human colon carcinoma cells in vitro
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کلمات کلیدی
MMCNDIMitomycin CFe-NTAcytokinesis-block micronucleus testROS - ROSHydrogen peroxide - آب اکسیژنهButyrate - بوتیراتmicronuclei - ریزهستک یا میکرونوکلئوس nuclear division index - شاخص تقسیم هسته ایferric nitrilotriacetate - فریت نیتریلتری اسیدH2O2 - هیدروژن پراکسیدHydrogen peroxide (H2O2) - پراکسید هیدروژن (H2O2)Reactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Butyrate, formed by bacterial fermentation of plant foods, has been shown to protect human colon cells from selected genotoxic substances. The mechanism for this effect could be the enhancement of toxicological defence leading to an increased detoxification of genotoxic risk factors and thus to a reduction of DNA and chromosome damage. Previous protective properties of butyrate against DNA damage induction in colon cells were demonstrated using the comet assay. In the present study the effect of butyrate on chromosome damage induced by ferric nitrilotriacetate (Fe-NTA) and hydrogen peroxide (H2O2) (suggested to be putative risk factors of colorectal carcinogenesis) was investigated using the cytokinesis-block micronucleus (CBMN) test. It was possible to reveal that pre-treatment of HT29 colon carcinoma cells with butyrate (2 and 4 mM) for 15 min caused a reduction of micronuclei induced with H2O2 (75 μM; p < 0.01) and Fe-NTA (500 and 1000 μM; p < 0.05). The decrease in the level of Fe-NTA- and H2O2-induced micronuclei was also confirmed in most of the corresponding variants of 24 h pre-treatment of cells with butyrate. The results obtained demonstrate for the first time protective properties of butyrate against chromosome damage induced by H2O2 and Fe-NTA in human colon carcinoma cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 23, Issue 6, September 2009, Pages 1028-1033
Journal: Toxicology in Vitro - Volume 23, Issue 6, September 2009, Pages 1028-1033
نویسندگان
Galina Hovhannisyan, Rouben Aroutiounian, Michael Glei,