کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2603200 1133812 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The roles of the thioredoxin system and peroxiredoxins in 1-methyl-4-phenyl-pyridinium ion-induced cytotoxicity in rat pheochromocytoma cells
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
The roles of the thioredoxin system and peroxiredoxins in 1-methyl-4-phenyl-pyridinium ion-induced cytotoxicity in rat pheochromocytoma cells
چکیده انگلیسی

The 1-methyl-4-phenyl-pyridinium ion (MPP+), an active metabolite of the neurotoxin, N-methyl-4-phenyl-1,2,3,6 tetrahydropyridine (MPTP), induces death in rat pheochromocytoma (PC12) cells, suggesting a cell model of Parkinson’s disease (PD). However, most of the toxic mechanisms remain illusive. In this study, we have found that MPP+ induced apoptotic cell death in PC12 cells as measured by the MTT assay and annexin V-FITC staining. Besides, MPP+ also resulted in decreased mitochondrial membrane potential and increased mitochondrial free radical formation as imaged by the staining of TMRE or MitoSOX, respectively, confirming the neurotoxic effect of MPP+ by interfering with mitochondrial functions. Western blot analysis indicated that MPP+ differentially regulated the expressions and over-oxidation of thioredoxin systems and peroxiredoxins. Since these enzymes are known to prevent oxidative stress and apoptosis, these evidences could be regarded as a novel neurotoxic mechanism of MPP+ and also provide an alternative view of developing drug therapies for PD.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 24, Issue 6, September 2010, Pages 1577–1583
نویسندگان
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