کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2603371 | 1133818 | 2008 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Oxidative stress might be a mechanism connected with the decreased α7 nicotinic receptor influenced by high-concentration of fluoride in SH-SY5Y neuroblastoma cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The possible mechanism concerning decreased α7 nicotinic acetylcholine receptor (nAChR) influenced by fluorosis was investigated. SH-SY5Y cells were exposed to fluoride within the range of 0.05-5 μM, or ferrous iron (1-100 μM), a free radical inducer. The levels of α7 nAChR expression, lipid peroxidation and protein oxidation were detected. The results showed that both high-concentrations of fluoride and ferrous iron induced increased levels of lipid peroxidation and protein oxidation in SH-SY5Y cells with concentration-dependent manners. In addition, inhibition of α7 nAChR at protein level was observed in the cells exposed to high amounts of fluoride or ferrous iron. Furthermore, a declined value of Bmax in [125I]α-bungarotoxin binding sites was found in the cells treated with the high-concentration of fluoride. Interestingly, antioxidants (vitamin E and glutathione) can attenuate the inhibition of the receptor induced by fluoride. These findings suggest that oxidative stress resulted from fluorosis might directly induce the deficit of α7 nAChR.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 22, Issue 4, June 2008, Pages 837-843
Journal: Toxicology in Vitro - Volume 22, Issue 4, June 2008, Pages 837-843
نویسندگان
Qin Gao, Yan-Jie Liu, Zhi-Zhong Guan,