کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2603531 1133823 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cobalt toxicity: Chemical and radiological combined effects on HaCaT keratinocyte cell line
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Cobalt toxicity: Chemical and radiological combined effects on HaCaT keratinocyte cell line
چکیده انگلیسی

Cobalt (Co) is an essential trace element well known as a constituent of vitamin B12, but different compounds of Co are also described as highly toxic and/or radiotoxic for individuals or the environment. In nuclear power plants, 58Co and 60Co are radioactive isotopes of cobalt present as activation products of stable Co and Ni used in alloys. Skin exposure is a current occupational risk in the hard metal and nuclear industries. As biochemical and molecular cobalt-induced toxicological mechanisms are not fully identified, we investigated cobalt toxicity in a model human keratinocyte cell line, HaCaT. In this study, we propose a model to determine the in vitro chemical impact on cell viability of a soluble form of cobalt (CoCl2) with or without γ-ray doses to mimic contamination by 60Co, to elucidate the mechanisms of cobalt intracellular chemical and radiological toxicity.Intracellular cobalt concentration was determined after HaCaT cell contamination and chemical toxicity was evaluated in terms of cellular viability and clonogenic survival. We investigated damage to DNA in HaCaT cells by combined treatment with chemical cobalt and a moderate γ-ray dose. Additive effects of cobalt and irradiation were demonstrated. The underlying mechanism of cobalt toxicity is not clearly established, but our results seem to indicate that the toxicity of Co(II) and of irradiation arises from production of reactive oxygen species.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 24, Issue 1, February 2010, Pages 92–98
نویسندگان
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