کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2604011 1133858 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
MIP-1β, a novel biomarker for in vitro sensitization test using human monocytic cell line
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
MIP-1β, a novel biomarker for in vitro sensitization test using human monocytic cell line
چکیده انگلیسی

In order to seek a novel biomarker for predicting skin sensitization, changes in the gene expression profile of THP-1 cells on exposure to 2,4-dinitrochlorobenzene (DNCB), p-phenylenediamine (pPD) and nickel sulfate (Ni) were assessed using oligo-DNA microarrays. While the change in gene expression varied depending on the sensitizers, up-regulation of MIP-1β mRNA expression was detected in both DNCB-treated and Ni-treated THP-1 cells. This finding was validated by RT-PCR and confirmed at the protein level by ELISA. Secretion of MIP-1β from THP-1 was detected after 24-h treatment with sensitizers such as DNCB, Ni, 2-mercaptobenzothiazole (2-MBT) and cobalt sulfate (Co), while pPD and non-sensitizers such as sodium dodecyl sulfate (SDS) and benzalkonium chloride (BC) had no effect. The use of both MIP-1β production and CD86 expression as criteria reduced the number of false-negatives, and the results were in good agreement with those of in vivo assays. MIP-1β may be useful as a novel biomarker in in vitro sensitization assay using THP-1 cells, either alone or in combination with known markers.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 20, Issue 5, August 2006, Pages 736–742
نویسندگان
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