Simple no-chromatography procedure for amine-reactive Eu3+ luminescent chelates optimal for bioconjugation

► Amine-reactive luminescent Eu3+ chelates were synthesized using simple no-chromatography procedure. ► The obtained chelates displayed high reactivity coupling to avidin at physiological conditions with high efficiency within a few minutes. ► The probes can find wide applications in assay development, imaging, as well as in proteomic studies that require high detection sensitivity.