کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2785676 | 1568381 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Resveratrol augments therapeutic efficiency of mouse bone marrow mesenchymal stem cell-based therapy in experimental autoimmune encephalomyelitis
ترجمه فارسی عنوان
رزوراترول افزایش کارآیی درمانی درمان مبتنی بر سلول های بنیادی مزانشیمی مغز استخوان موش ها در آنسفالومیلیت های اتوایمیون تجربی
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کلمات کلیدی
IFN-γIL-4EAEIL-10experimental autoimmune encephalitis - آنسفالیت autoimmune تجربیinterleukin-4 - اینترلوکین -4Interleukin-10 - اینترلوکین 10ELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاtumor necrosis factor alpha - تومور نکروز عامل آلفاCNS - دستگاه عصبی مرکزیResveratrol - رسوراترولMesenchymal stem cells - سلول های بنیادی مزانشیمیcentral nervous system - سیستم عصبی مرکزیTNF-α - فاکتور نکروز توموری آلفاMultiple sclerosis - مولتیپل اسکلروزیس(ام اس)Interferon gamma - گاما اینترفرون
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
چکیده انگلیسی
Experimental autoimmune encephalitis (EAE) is an inflammatory demyelinating disease, which served as a useful model providing considerable insights into the pathogenesis of multiple sclerosis (MS). Mouse bone marrow mesenchymal stem cells (mBM-MSC) were shown to have neuroprotection capabilities in EAE. Resveratrol is a small polyphenolic compound and possess therapeutic activity in various immune-mediated diseases. The sensitivity of mBM-MSCs to resveratrol was determined by an established cell-viability assay. Resveratrol-treated mBM-MSCs were also characterized with flow cytometry using MSC-specific surface markers and analyzed for their multiple differentiation capacities. EAE was induced in C57BL/6 mice by immunization with MOG35-55. Interferon gamma (IFN-γ)/tumor necrosis factor alpha (TNF-α) and interleukin-4 (IL-4)/interleukin-10 (IL-10), the hallmark cytokines that direct T helper type 1 (Th1) and Th2 development, were detected with enzyme-linked immunosorbent assay (ELISA). In vivo efficacy experiments showed that mBM-MSCs or resveratrol alone led to a significant reduction in clinical scores, and combined treatment resulted in even more prominent reduction. The combined treatment with mBM-MSCs and resveratrol enhanced the immunomodulatory effects, showing suppressed proinflammatory cytokines (IFN-γ, TNF-α) and increased anti-inflammatory cytokines (IL-4, IL-10). The combination of mBM-MSCs and resveratrol provides a novel potential experimental protocol for alleviating EAE symptoms.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Developmental Neuroscience - Volume 49, April 2016, Pages 60-66
Journal: International Journal of Developmental Neuroscience - Volume 49, April 2016, Pages 60-66
نویسندگان
Dong Wang, Shi-Ping Li, Jin-Sheng Fu, Lin Bai, Li Guo,