کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2819298 | 1569918 | 2008 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of the avian infectious bronchitis coronaviruses with mutations in gene 3
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کلمات کلیدی
EID50EnvelopTOCIBVORFnucleocapsidSARS-CoVTRSSpike - اسپایکInfectious bronchitis - برونشیت عفونیmutation - جهشreverse transcription - رونویسی معکوسGene order - سفارش ژنMembrane - غشاءnonstructural - غیر ساختاریopen reading frame - قاب خواندن بازInfectious bronchitis coronavirus - قرنیه ویروس برونشیت عفونیReplication - همانندسازی polymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازInfectious bronchitis virus - ویروس برونشیت عفونیPathogenicity - پاتوژنزSevere acute respiratory syndrome-associated coronavirus - کرونا ویروس مرتبط با سندرم حاد تنفسی شدید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Identification of the avian infectious bronchitis coronaviruses with mutations in gene 3 Identification of the avian infectious bronchitis coronaviruses with mutations in gene 3](/preview/png/2819298.png)
چکیده انگلیسی
The sequence of a 6.0-kb fragment was compared in the 3â²-encoding region of the genome in 27 infectious bronchitis virus (IBV) strains. All these strains have the same S-3-M-5-N gene order, as is the case for other IBVs. However, the sizes of the corresponding open reading frames (ORFs) of some genes varied among the virus strains. Phylogenetic analysis and sequence alignments demonstrated that recombination events had occurred in the origin and evolution of the strains CK/CH/LSD/03I and CK/CH/LLN/98I and the possible recombinant junction sites might be located at the 3c and M genes, respectively. The normal product of ORF 3a is 57 amino acids long, whereas a 43-bp deletion at the 3â²-end of the CK/CH/LSD/03I 3a gene was detected, resulting in a frameshift event and C-terminally truncated protein with 47 amino acids. Comparison of the growth ability in embryos and replication and pathogenicity in chickens with IBV carrying the normal 3a gene indicated that this deleted sequence in the 3a gene of CK/CH/LSD/03I was not necessary for viral pathogenesis and replication either in vitro or in vivo. Occurrence of a mutation at the corresponding position of the CK/CH/LLN/98I start codon in the 3a gene led to the absence of ORF 3a in this virus, resulting in a novel genomic organization at the 3â²-encoding regions: S-3b, 3c-M-5a, 5b-N. Comparison with other viruses carrying the normal 3a gene revealed that CK/CH/LLN/98I had replication and pathogenicity abilities in vivo similar to those of other IBVs; however, its growth ability in embryos was lower, although the relationship between the lower growth ability and the ORF 3a defect requires further confirmation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 412, Issues 1â2, 15 April 2008, Pages 12-25
Journal: Gene - Volume 412, Issues 1â2, 15 April 2008, Pages 12-25
نویسندگان
Shengwang Liu, Qingxia Zhang, Jianfei Chen, Zongxi Han, Yuhao Shao, Xiangang Kong, Guangzhi Tong,