کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2831158 1163782 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Folate deficiency enhances the inflammatory response of macrophages
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Folate deficiency enhances the inflammatory response of macrophages
چکیده انگلیسی

B-vitamin deficiency is a risk factor for vascular disease. The mechanism by which the deficiency impacts on disease risk is unclear. We have analysed whether the inflammatory response of mononuclear cells can be modified by cellular folate status in vitro.We show that the mouse monocyte cell line RAW264.7 grown under folate restriction displays a decrease in intracellular folate levels and a reduced growth rate. The cells also show a 2- to 3-fold increase in expression of the inflammatory mediators, IL1β, IL6, TNFα and MCP1 at the RNA and protein level (p < 0.01) under conditions of folate deficiency. In contrast the production of the vaso-protective mediator nitric oxide is significantly reduced under these conditions. These metabolic changes are independent of the concentration of homocysteine in the medium and occur in the absence of significant changes in global DNA methylation. Folate deficiency may therefore exacerbate cardiovascular disease by augmenting pro-inflammatory signals in the monocyte-macrophage lineage.


► B-vitamin deficiency is a risk factor for vascular disease.
► The mechanism by which the deficiency impacts on disease risk is unclear.
► B-vitamin deficiency promotes the expression of pro-inflammatory cytokines.
► B-vitamin deficiency reduces nitric oxide generation.
► These changes occur in the absence of increases in extra-cellular homocysteine.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 54, Issue 2, June 2013, Pages 164–172
نویسندگان
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