کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
29197 | 44130 | 2006 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Coherent control improves biomedical imaging with ultrashort shaped pulses Coherent control improves biomedical imaging with ultrashort shaped pulses](/preview/png/29197.png)
Although ultrashort pulses are advantageous for multiphoton excitation microscopy, they can be difficult to manipulate and may cause increased sample damage when applied to biological tissue. Here we present a method based on coherent control that corrects phase distortions introduced by high numerical aperture (NA) microscope objectives, thereby achieving the full potential of ultrashort pulses. A number of useful phase functions are recommended to gain selectivity that is similar to that which can be achieved by tuning a longer laser pulse; however this one involves no moving parts and maintains perfect optimization. This capability is used to demonstrate functional imaging by selective two-photon excitation of a pH-sensitive chromophore. Finally, we show that phase functions can also be introduced to minimize multiphoton excitation damage, while maintaining a high efficiency of two-photon excitation.
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 180, Issue 3, 25 June 2006, Pages 307–313