کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2928928 1576155 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ultrastructural changes associated with myocardial apoptosis, in failing rat hearts induced by volume overload
ترجمه فارسی عنوان
تغییرات فوق العاده ساختاری مرتبط با آپوپتوز قلب، در شکستگی قلب موش ناشی از اضافه بار حجم
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
چکیده انگلیسی

BackgroundMyocardial apoptosis has been discussed to play a pivotal role in the development and progression of congestive heart failure (CHF). However, recently there is doubt on the evidence of myocardial apoptosis in heart failure as information on ultrastructural changes by electron microscopy is still scarce. This project therefore aimed to detect direct morphological evidence of myocardial apoptosis in an experimental heart failure model.MethodFollowing IRB approval, an aortocaval fistula (ACF) was induced in male Wistar rats using a 16G needle. 28 ± 2 days following ACF rats were examined by hemodynamic measurements, Western blot, immunofluorescence confocal and electron microscopic analysis.ResultsWithin 28 ± 2 days of ACF heart (3.8 ± 0.1 vs. 6.6 ± 0.3 mg/g) and lung (3.7 ± 0.2 vs. 6.9 ± 0.5 mg/g) weight indices significantly increased in the ACF group accompanied by a restriction in systolic (LVEF: 72 ± 2 vs. 39 ± 3%) and diastolic (dP/dtmin.: − 10,435 ± 942 vs. − 5982 ± 745 mmHg/s) function (p < 0.01). Activated caspase-3 was significantly increased in failing hearts concomitant with mitochondrial leakage of cytochrome c into the cytosol. Finally, electron microscopy of the left ventricle (LV) of ACF rats revealed pronounced ultrastructural changes in > 70% of examined cardiomyocytes, such as nuclear chromatin condensation, myofibril loss and disarray, contour irregularities and amorphous dense bodies, mitochondriosis and damaged cell–cell-contacts between cardiomyocytes.ConclusionsVolume overload induced heart failure is associated with activation of the mitochondrial apoptotic pathway. In addition, electron microscopy of the LV revealed direct ultrastructural evidence of extended myocardial apoptosis in ACF rats.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Cardiology - Volume 197, 15 October 2015, Pages 327–332
نویسندگان
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