کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3029475 | 1183078 | 2008 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Alteration in protein kinase B (AKT) activity in platelets from patients with systemic sclerosis
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کلمات کلیدی
eNOSpH 7.4PI 3-KTMBTBSTPrPECLTween 20vWFSSCcGMPAkt - آکتsystemic sclerosis - اسکلروز سیستمیکenhanced chemiluminescence - بهبود شیمیایی لومنtetramethylbenzidine - تترامتیل بنزیدینEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاVon Willebrand factor - عامل فون ویلبراندPhosphatidylinositol 3-kinase - فسفاتیدیلینواستیل 3-کینازNitric oxide - نیتریک اکسیدnitric oxide synthase - نیتریک اکسید سنتازprotein kinase B - پروتئین کیناز Bplatelet-rich plasma - پلاسمای غنی از پلاکت، PRP، پی آر پیPlatelet - پلاکتCollagen - کلاژنglycoprotein IIb/IIIa - گلیکوپروتئین IIb / IIIa
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
کاردیولوژی و پزشکی قلب و عروق
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چکیده انگلیسی
We have previously reported that there is an increase in phosphotidylinositide-3 kinase activity secondary to increase in nitrotyrosylation in platelets from patients with SSc. Protein kinase B (Akt) is recruited to the plasma membrane by PI 3-K metabolites. Both enzymes play critical roles in signal transduction and activation of platelets. In the present investigation, we have studied the effect of postranslational modification of the activity of Akt. We have examined eight patients and eight controls and obtained results showing that enzymatic activity of Akt is increased in lysates of platelets from patients with SSc compared to normal volunteer controls. We have obtained results showing that there is no correlation of nitrotyrosylation of Akt on its enzymatic activity although Western blots show the enzyme has increased nitrotyrosylation. These results suggest that post-translational modification by nitrotyrosylation does not control Akt in SSc platelets. We concluded that the enhanced activity of PI 3-K and Akt in platelets from patients with SSc is mediated by different mechanisms.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Thrombosis Research - Volume 122, Issue 4, 2008, Pages 501-506
Journal: Thrombosis Research - Volume 122, Issue 4, 2008, Pages 501-506
نویسندگان
Thomas M. Chiang, Arnold E. Postlethwaite,