کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3029896 | 1183124 | 2007 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A segment of Staphylococcus aureus clumping factor A with fibrinogen-binding activity (ClfA221-550) inhibits platelet-plug formation in mice
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کلمات کلیدی
APTTGSTTBSclfAAntithrombotic - آنتی ترومبوتیکSodium dodecylsulfate-polyacrylamide gel electrophoresis - الکتروفورز ژل سدیم دودسیل سولفات - پلی آکریل آمیدSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدTris-buffered saline - تریس بافر شورactivated partial thromboplastin time - زمان ترومبوپلاستین جزئی فعال شده استprothrombin time - زمان پروترومبینclumping factor A - فشردگی فاکتور APlatelet - پلاکتglutathione S-transferase - گلوتاتیون S-ترانسفرازGlycoprotein - گلیکوپروتئین
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
کاردیولوژی و پزشکی قلب و عروق
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A segment of Staphylococcus aureus clumping factor A with fibrinogen-binding activity (ClfA221-550) inhibits platelet-plug formation in mice A segment of Staphylococcus aureus clumping factor A with fibrinogen-binding activity (ClfA221-550) inhibits platelet-plug formation in mice](/preview/png/3029896.png)
چکیده انگلیسی
We previously reported that the fibrinogen-binding segment (residues 221-550) of Staphylococcus aureus clumping factor A (ClfA), which binds to fibrinogen γ chain C-terminus, exerted inhibitory effects on platelet aggregation and fibrin clot formation in vitro. Here, we further demonstrated the effectiveness of using ClfA221-550 to inhibit platelet-rich thrombus formation in vivo. Platelet-rich thrombi were formed in the mesenteric venules of fluorescein-loaded mice by filtered light illumination. It grew rapidly and ultimately resulted in the cessation of blood flow due to vessel occlusion. Given by intravenous bolus injection, ClfA221-550 delayed occlusive thrombi formation in a dose-dependent manner: 2-, 3- and 4.5-fold prolongations of vessel occlusion time were attained with 0.69, 6.9 and 34.5 mg/kg of ClfA221-550, respectively. Reduced fibrin clot formation at the late phase with plasmas, which were prepared from ClfA221-550-treated mice, was also dose-dependent. The suppression of fibrin formation ex vivo coincided with the delay of occlusive thrombus formation in vivo, suggesting that the antithrombotic effect of ClfA221-550 may result from the blockade of fibrinogen γ chain C-terminal functions, in mediating platelet aggregation and fibrin clot formation. Administration of ClfA221-550 also lengthened the tail bleeding of mice; however, significant effect was achieved only with a higher dosage, namely 34.5 mg/kg. These results together showed that blockade of fibrinogen γ chain C-terminus with ClfA221-550 preferentially affected platelet-rich thrombus formation rather than normal haemostasis, thus providing a rationale for selecting fibrinogen γ chain C-terminus as a new target for thrombotic intervention.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Thrombosis Research - Volume 121, Issue 2, 2007, Pages 183-191
Journal: Thrombosis Research - Volume 121, Issue 2, 2007, Pages 183-191
نویسندگان
Chao-Zong Liu, Tur-Fu Huang, Po-Jun Tsai, Pei-Jane Tsai, Ling-Ya Chang, Mei-Chi Chang,