کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3055313 1186468 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Activation of LILRB2 signal pathway in temporal lobe epilepsy patients and in a pilocarpine induced epilepsy model
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی عصب شناسی
پیش نمایش صفحه اول مقاله
Activation of LILRB2 signal pathway in temporal lobe epilepsy patients and in a pilocarpine induced epilepsy model
چکیده انگلیسی


• The expression of LILRB2/PirB in patients with intractable TLE and model mice was dramatically increased, respectively.
• The downstream molecules of LILRB2/PirB have been activated in the epileptic foci of TLE patients.
• LILRB2/PirB and its downstream factors are highly expressed in neurons and astrocytes.
• The protein levels of LILRB2 negatively correlated with the frequency of seizures in TLE patients.

Temporal lobe epilepsy (TLE) is a frequent form of focal intractable epilepsy in adults, but the specific mechanism underlying the epileptogenesis of TLE is still unknown. Human leukocyte immunoglobulin-like receptor B2 (LILRB2) (the murine homolog gene called paired immunoglobulin-like receptor B, or PirB), participates in the process of synaptic plasticity and neurite growth in the central nervous system (CNS), suggesting a potential role of LILRB2 in epilepsy. However, the expression pattern of LILRB2 and the downstream molecular signal in intractable TLE remains poorly understood. In the present study, western blotting and immunohistochemistry results showed that LILRB2 expression was upregulated in the temporal neocortex of patients with TLE. Moreover, protein levels of LILRB2 negatively correlated with the frequency of seizures in TLE patients. In the pilocarpine-induced C57BL/6 mouse model, PirB upregulation in the hippocampus began 12 h after status epilepticus (SE), reached a peak at 7 days and then maintained a significantly high level until day 60. Similarly, we found a remarkable increase in PirB expression at 1 day, 7 days and30days post-SE in the temporal cortex. Double-labeled immunofluorescence showed that LILRB2/PirB were highly expressed in neurons and astrocytes but not microglia. In addition, protein levels of POSH, SHROOM3, ROCK1 and ROCK2, the important downstream factors of the LILRB2 pathway, were significantly increased in the epileptic foci of TLE patients and located on the NeuN-positive neurons and GFAP-positive astrocytes. Taken together, our results indicate that LILRB2/PirB may be involved in the process of TLE.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Neurology - Volume 285, Part A, November 2016, Pages 51–60
نویسندگان
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