Do first order and higher order regions of the thalamic reticular nucleus have different developmental timetables?

The thalamic reticular nucleus (TRN) can been subdivided into sectors based on thalamic and cortical input. Additionally, in carnivores the visual sector of the TRN can be subdivided into first order (perigeniculate nucleus: PGN) and higher order (TRN) regions. This report examines whether TRN development reflects the nature of its higher order visual connections. 170 cells from 12 kittens aged between postnatal day 0 (P0) and P125 were fully analysed after single cell injections in 400-500 μm fixed brain slices. TRN cells have a period of exuberant dendritic branching that peaks between P3 and P12, around the time of eye opening (P7), followed by branch pruning until P68. Similarly, most dendritic appendages are added between P12 and P22 followed by pruning, which is also largely complete by P68. Most branch points occur within the first 10-30% of the dendritic arbor, peaking between 10 and 20% (roughly equivalent to 100 μm from the soma), while appendages were concentrated between 20 and 30% of the arbour; appendages tend to be distributed over a larger proportion of the arbor up to P14 compared to later ages. TRN and PGN maturation were not significantly different. The present data suggest that clear distinctions cannot be made between the maturation of first and higher order pathways and indicate that GABAergic cells of the ventral thalamus may mature earlier than relay cells of the dorsal thalamus. Furthermore, dendritic development in the TRN may be less dependent on extrinsic factors than an intrinsic growth pattern or factors other than a functional hierarchy within the visual pathway.