کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3064452 | 1580443 | 2011 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression of the α7 nAChR subunit duplicate form (CHRFAM7A) is down-regulated in the monocytic cell line THP-1 on treatment with LPS
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کلمات کلیدی
EtOH, ethanol - EtOH، ethanolGAPDH, glyceraldehyde-3-phosphate dehydrogenase - GAPDH، glyceraldehyde-3-phosphate dehydrogenasenAChR, nicotinic acetylcholine receptor - nAChR، گیرنده استیل کولین نیکوتینTNF?, tumor necrosis factor ? - TNF؟ عامل فاکتور نکروز تومورDMSO, dimethyl sulfoxide - دیمتیل سولفواکسیدPBS, phosphate buffered saline - محلول نمکِ فسفات با خاصیت بافریLPS, lipopolysaccharide - مولکولهای لیپوپلی ساکارید
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Expression of the α7 nAChR subunit duplicate form (CHRFAM7A) is down-regulated in the monocytic cell line THP-1 on treatment with LPS Expression of the α7 nAChR subunit duplicate form (CHRFAM7A) is down-regulated in the monocytic cell line THP-1 on treatment with LPS](/preview/png/3064452.png)
چکیده انگلیسی
We investigated the expression of the partially duplicated α7 nAChR subunit gene in three monocytic cell lines: THP-1, U937 and Mono-Mac-6. Qualitative PCR revealed the presence of the classic α7 gene in these lineages, but real-time PCR showed the exclusive expression of α7dup. Both mRNA and protein levels were reduced in THP-1 upon LPS challenge, and it was found that transcriptional down-regulation was mediated by a direct mechanism dependent on NF-κB as its specific inhibitor parthenolide prevented the reduction in the α7dup transcript. Such precise regulation suggests that α7dup may specifically participate in the inflammatory response of the innate immune system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroimmunology - Volume 230, Issues 1–2, January 2011, Pages 74–84
Journal: Journal of Neuroimmunology - Volume 230, Issues 1–2, January 2011, Pages 74–84
نویسندگان
Roberta Benfante, Ruth Adele Antonini, Maria De Pizzol, Cecilia Gotti, Francesco Clementi, Massimo Locati, Diego Fornasari,