کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3069620 1580698 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Monocarboxylate transporter 1 is deficient on microvessels in the human epileptogenic hippocampus
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی عصب شناسی
پیش نمایش صفحه اول مقاله
Monocarboxylate transporter 1 is deficient on microvessels in the human epileptogenic hippocampus
چکیده انگلیسی

Monocarboxylate transporter 1 (MCT1) facilitates the transport of important metabolic fuels (lactate, pyruvate and ketone bodies) and possibly also acidic drugs such as valproic acid across the blood–brain barrier. Because an impaired brain energy metabolism and resistance to antiepileptic drugs are common features of temporal lobe epilepsy (TLE), we sought to study the expression of MCT1 in the brain of patients with this disease. Immunohistochemistry and immunogold electron microscopy were used to assess the distribution of MCT1 in brain specimens from patients with TLE and concomitant hippocampal sclerosis (referred to as mesial TLE or MTLE (n = 15)), patients with TLE and no hippocampal sclerosis (non-MTLE, n = 13) and neurologically normal autopsy subjects (n = 8). MCT1 was present on an extensive network of microvessels throughout the hippocampal formation in autopsy controls and to a lesser degree in non-MTLE. Patients with MTLE were markedly deficient in MCT1 on microvessels in several areas of the hippocampal formation, especially CA1, which exhibited a 37% to 48% loss of MCT1 on the plasma membrane of endothelial cells when compared with non-MTLE. These findings suggest that the uptake of blood-derived monocarboxylate fuels and possibly also acidic drugs, such as valproic acid, is perturbed in the epileptogenic hippocampus, particularly in MTLE. We hypothesize that the loss of MCT1 on brain microvessels is mechanistically involved in the pathophysiology of drug-resistant TLE, and propose that re-expression of MCT1 may represent a novel therapeutic approach for this disease.

Research Highlights
► Monocarboxylate transporter 1 (MCT1) is highly enriched on brain microvessels.
► MCT1 carries metabolic fuels and acidic drugs across the blood brain barrier.
► MCT1 is deficient on brain microvessels in mesial temporal lobe epilepsy (MTLE)
► This deficiency may be implicated in the causation of drug-resistant MTLE.
► MCT1 may represent a novel therapeutic target for patients with this disease.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurobiology of Disease - Volume 41, Issue 2, February 2011, Pages 577–584
نویسندگان
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