کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
31567 44819 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Urease expression in a Thermoanaerobacterium saccharolyticum ethanologen allows high titer ethanol production
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Urease expression in a Thermoanaerobacterium saccharolyticum ethanologen allows high titer ethanol production
چکیده انگلیسی

Genes encoding the enzyme urease were integrated in a Thermoanaerobacterium saccharolyticum ethanologen. The engineered strain hydrolyzed urea, as evidenced by increased cellular growth and elevated final pH in urea minimal medium and urease activity in cell free extracts. Interestingly, replacement of ammonium salts with urea resulted in production of 54 g/L ethanol, one of the highest titers reported for Thermoanaerobacterium. The observed increase in ethanol titer may result from reduced pH, salt, and osmolality stresses during fermentation. Urea utilization is attractive for industrial scale fermentation, where pH control is technically challenging and increased ethanol titer is desirable.


► Genes encoding urease were integrated in a T. saccharolyticum ethanologen.
► Replacement of ammonium salts with urea resulted in production of 54 g/L ethanol.
► Urea utilization is attractive for industrial scale fermentation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Metabolic Engineering - Volume 14, Issue 5, September 2012, Pages 528–532
نویسندگان
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