کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
31600 | 44823 | 2013 | 15 صفحه PDF | دانلود رایگان |
The interactions between the intracellular metabolome, fluxome and growth rate of Escherichia coli after sudden glycolytic/gluconeogenic substrate shifts are studied based on pulses of different substrates to an aerobic glucose-limited steady-state (dilution rate=0.1 h−1). After each added glycolytic (glucose) and gluconeogenic (pyruvate and succinate) substrate pulse, no by-products were secreted and a pseudo steady state in flux and metabolites was achieved in about 30–40 s. In the pulse experiments a large oxygen uptake capacity of the cells was observed. The in vivo dynamic responses showed massive reorganization and flexibility (1/100–14-fold change) of extra/intracellular metabolic fluxes, matching with large changes in the concentrations of intracellular metabolites, including reversal of reaction rate for pseudo/near equilibrium reactions. The coupling of metabolome and fluxome could be described by Q-linear kinetics. Remarkably, the three different substrate pulses resulted in a very similar increase in growth rate (0.13–0.3 h−1). Data analysis showed that there must exist as yet unknown mechanisms which couple the protein synthesis rate to changes in central metabolites.
► Different substrate pulses lead to 2.3-fold increase in the growth rate of E. coli.
► There is a yet unknown regulation mechanism of growth rate in E. coli.
► Different substrate pulses expose a high flexibility of central metabolic fluxes.
► In vivo parameters of reactions in E. coli were deduced by Q-linear kinetics.
Journal: Metabolic Engineering - Volume 16, March 2013, Pages 115–129