Development and characterization of salivary adenoid cystic carcinoma cell line

SummaryObjectiveTo develop in vitro adenoid cystic carcinoma cell line as a surrogate for functional studies.Materials and methodsCells obtained from a primary ACC of the base of tongue were cultivated in vitro and immortalized with h-TERT. Morphologic, cytogenetic and functional studies were performed.ResultsTumor cells were verified by positive reactions to keratin and smooth muscle actin and phenotypic cellular and nuclear features. In-vitro cell growth and colony formation assay supported their tumor nature.ConclusionWe authenticated an ACC cell line with hybrid epithelial–myoepithelial feature as a resource for functional experimentation.