کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3256954 | 1207380 | 2013 | 13 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Unexpected heterogeneity of multifunctional T cells in response to superantigen stimulation in humans Unexpected heterogeneity of multifunctional T cells in response to superantigen stimulation in humans](/preview/png/3256954.png)
Toxic shock syndrome (TSS) is a potentially life threatening condition characterized by fever, rash, shock, and multi-organ failure. Staphylococcal enterotoxin B (SEB) is a well characterized superantigen that has been shown to play an important role in TSS. Although the precise mechanisms by which SEB and other superantigens cause TSS are unknown, induction of a pro-inflammatory cytokine cascade appears central to this phenomenon. We show that CD4 + and CD8 + Teffector/memory (TEM) and other subsets produce IL-17A following SEB stimulation. We also show that IL-17A is co-produced with other pro-inflammatory cytokines (i.e., IL-2, IFN-γ and TNF-α). These responses are significantly different than those elicited by mitogenic stimulation. Multifunctional IL-17A producing cells possess markers typical of the TH17/TC17 and TH1 subsets, including CCR6, IL-22, and transcription factors retinoic acid receptor-related orphan nuclear receptor (ROR)-γt and T-bet. These results suggest a possible role for IL-17A-producing multifunctional T cells in the pathogenesis of TSS.
► We studied secretion of IL-17A by CD4 + and CD8 + T cells to direct TCR stimulation.
► IL-17A-secreting T cells are multifunctional, co-expressing IFN-γ, TNF-α and IL-2.
► Multifunctional IL-17A + cells express markers of TH17/TC17 and TH1 subsets.
► Unsupervised FLOCK analysis uncovered additional distinct IL-17A + populations.
► IL-17A multifunctional T cells are likely to play a role in the pathogenesis of TSS.
Journal: Clinical Immunology - Volume 146, Issue 2, February 2013, Pages 140–152