کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3256995 | 1207384 | 2012 | 11 صفحه PDF | دانلود رایگان |

Noroviruses are the most frequent cause of acute gastroenteritis in humans of all ages. No vaccines are currently available. An intranasally delivered Norwalk (NV) virus-like particle (VLP) vaccine was recently shown to be well tolerated, immunogenic and to protect against infection in Phase 1 studies. Here, we examined B memory (BM) responses in volunteers who received the highest dosage levels of the NV-VLP vaccine (50 μg and 100 μg). We measured the frequency of NV-specific IgG and IgA-secreting BM cells in peripheral blood and the level of antibodies produced by these cells in culture. All subjects immunized with 100 μg of the NV-VLP vaccine and 90% of those who received 50 μg had significant IgA or IgG BM responses. The BM cell frequencies correlated with serum antibody levels and mucosally-primed antibody-secreting cell responses. This is the first demonstration of dose-dependent, functional BM responses in humans immunized intranasally with a NV-VLP vaccine.
► We studied B memory cells in humans immunized with Norwalk virus-like particles.
► BM cell frequency was measured in blood from volunteers immunized intranasally.
► All subjects that received 100 μg of VLP developed IgG and IgA-secreting BM cells.
► BM cell responses correlated with serum antibodies and antibody secreting cells.
► This is the first report of human BM responses to an intranasal Norwalk VLP vaccine.
Journal: Clinical Immunology - Volume 144, Issue 2, August 2012, Pages 98–108