کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3257176 | 1207395 | 2012 | 11 صفحه PDF | دانلود رایگان |

Systemic juvenile idiopathic arthritis (SJIA) is a chronic autoinflammatory condition. The association with macrophage activation syndrome, and the therapeutic efficacy of inhibiting monocyte-derived cytokines, has implicated these cells in SJIA pathogenesis. To characterize the activation state (classical/M1 vs. alternative/M2) of SJIA monocytes, we immunophenotyped monocytes using several approaches. Monocyte transcripts were analyzed by microarray and quantitative PCR. Surface proteins were measured at the single cell level using flow cytometry. Cytokine production was evaluated by intracellular staining and ELISA. CD14++CD16− and CD14+CD16+ monocyte subsets are activated in SJIA. A mixed M1/M2 activation phenotype is apparent at the single cell level, especially during flare. Consistent with an M2 phenotype, SJIA monocytes produce IL-1β after LPS exposure, but do not secrete it. Despite the inflammatory nature of active SJIA, circulating monocytes demonstrate significant anti-inflammatory features. The persistence of some of these phenotypes during clinically inactive disease argues that this state reflects compensated inflammation.
► Monocytes are implicated in SJIA pathogenesis based on clinical and mechanistic data.
► We characterized the activation phenotype of SJIA monocytes during flare and quiescence.
► SJIA monocytes, especially during flare, show a mixed M1/M2-like activation phenotype.
► During quiescence SJIA monocytes tend to exhibit a M2-like phenotype.
► Circulating monocytes may play a pro- but also an anti-inflammatory role in SJIA.
Journal: Clinical Immunology - Volume 142, Issue 3, March 2012, Pages 362–372