کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3257240 | 1589431 | 2011 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Induction of type B synoviocyte-like cells from plasmacytoid dendritic cells of the bone marrow in rheumatoid arthritis and osteoarthritis Induction of type B synoviocyte-like cells from plasmacytoid dendritic cells of the bone marrow in rheumatoid arthritis and osteoarthritis](/preview/png/3257240.png)
We examined the capacities of bone marrow (BM) plasmacytoid dendritic cells (pDC) to differentiate into type B synoviocyte-like cells. BM aspiration samples were obtained from 24 rheumatoid arthritis (RA) patients and 19 osteoarthritis (OA) patients during joint operations from the iliac crest. CD34+ cells and pDC purified from BM mononuclear cells were cultured with or without SCF, GM-CSF, and TNF-α for 2–4 weeks. RA BM pDC as well as OA BM pDC comparably differentiated into fibroblast-like cells (FLC), expressing cadherin-11 and producing MMP-1, especially in the presence of TNF-α. Of note, depletion of BDCA4+ pDC from RA BM CD34+ cells significantly diminished their capacities to differentiate into FLC, which were restored by addition of BDCA4 + cells in a dose–response manner. These results indicate that pDC is one of the progenitors of type B synoviocytes, suggesting that BM pDC might be involved in the pathogenesis of RA and OA.
Research highlights
► RA BM pDC as well as OA BM pDC comparably differentiated into fibroblast-like cells (FLC).
► Depletion of BDCA4+ pDC from RA BM CD34+ cells diminished their capacities to differentiate into FLC.
► Addition of BDCA4+cells restored their capacities to differentiate into FLC in a dose–response manner.
► These results indicate that pDC is one of the progenitors of type B synoviocytes.
► The data suggest that BM pDC might be involved in the pathogenesis of RA and OA.
Journal: Clinical Immunology - Volume 140, Issue 3, September 2011, Pages 276–283