کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3257339 | 1207407 | 2009 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Differential dynamics of donor DC and non-DC peripheral blood mononuclear cell microchimerism in lung transplantation Differential dynamics of donor DC and non-DC peripheral blood mononuclear cell microchimerism in lung transplantation](/preview/png/3257339.png)
Donor cell microchimerism induces tolerance in animal models and may increase graft survival in man. Since dendritic cells (DC) are critical for induction of both tolerance and alloreactivity we developed a method to quantitate microchimerism in donor DC and non-DC in peripheral blood mononuclear cells (PBMC) after lung transplantation. Longitudinal analysis of donor cell microchimerism in eleven sex mismatched lung transplant recipients (LTR) up to 12 months post-transplant used Y chromosome based real-time PCR on sorted cells. Total DC or a proportion of DC subsets in PBMC did not change but there were heterogeneous and dynamic changes in microchimerism in DC and non-DC. Analysis of changes in DC using a mixed model analysis showed significantly less reduction in DC compared to non-DC over time (0.49, p = 0.001). Preferential DC persistence compared to non-DC may indicate tolerance induction but future studies are required to determine if DC microchimerism after transplantation affects clinical outcomes.
Journal: Clinical Immunology - Volume 133, Issue 2, November 2009, Pages 179–183