کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3257385 1207411 2011 15 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Human T cell development in the liver of humanized NOD/SCID/IL-2Rγnull(NSG) mice generated by intrahepatic injection of CD34+ human (h) cord blood (CB) cells
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Human T cell development in the liver of humanized NOD/SCID/IL-2Rγnull(NSG) mice generated by intrahepatic injection of CD34+ human (h) cord blood (CB) cells
چکیده انگلیسی

In this study, we explore the possibility of human T cell development in the liver of humanized mice generated by intrahepatic injection of CD34+ hCB cells into conditioned NOD/SCID/IL-2Rγnull(NSG) newborn mice. The intrahepatic injection of CD34+ hCB cells led to effective reconstitution of human myeloid and lymphoid lineage cells. In contrast to the previously reported Rag2−/−γc−/− humanized mice, interestingly, the thymus function of humanized NSG mice was markedly reduced in terms of its size and cell contents, whereas the livers of humanized NSG mice profoundly contained double-positive (DP), hCD4 and hCD8 single positive (SP), hCD34+hCD38lohCD1a− (TSP), hCD34+hCD38hihCD1a− (ETP), and hCD34+hCD38+hCD1a+ (pre-T cells) cells. Furthermore, immunostaining of the liver revealed that human T cells were co-localized with hDCs. Taken together, our results demonstrate that the intrahepatic injection of hCD34+ hCB cells can facilitate human T cell development in the livers of humanized NSG mice.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinical Immunology - Volume 139, Issue 3, June 2011, Pages 321–335
نویسندگان
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