کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3257522 | 1207419 | 2010 | 13 صفحه PDF | دانلود رایگان |
Murine retinal pigment epithelial (RPE) cells suppress T-cell activation by releasing soluble inhibitory factors and promote the generation of regulatory T cells in vitro. These T cells exposed to RPE supernatants (RPE-induced Treg cells) can suppress the activation of bystander effector T cells via the production of transforming growth factor-beta (TGFβ). In the present study, we showed that human RPE-induced Treg cells are also able to acquire regulatory function when human RPE cell lines were pretreated with recombinant TGFβ2. These RPE-induced Treg cells produced TGFβ1 and IL-10 but not IFNγ, and they significantly suppressed the activation of target cell lines and intraocular T-cell clones established from patients with active uveitis. Moreover, CD4+CD25+ RPE-induced Treg cells expressed CTLA-4 and Foxp3 molecules, and the CD25+ Treg cells profoundly suppressed the T-cell activation. Thus, in vitro manipulated Treg cells acquire functions that participate in the establishment of immune tolerance in the eye.
Journal: Clinical Immunology - Volume 136, Issue 1, July 2010, Pages 83–95