کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3257771 | 1207424 | 2010 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Experimental extracorporeal photopheresis therapy significantly delays the development of diabetes in non-obese diabetic mice Experimental extracorporeal photopheresis therapy significantly delays the development of diabetes in non-obese diabetic mice](/preview/png/3257771.png)
In our previous studies, we demonstrated that infusion of apoptotic cells significantly prevented type 1 diabetes (T1D) in non-obese diabetic (NOD) mice. Extracorporeal photopheresis (ECP) is an apoptotic cell-based therapy used clinically for immune-mediated disorders. In this study, we examined the effect that intravenous delivery of apoptotic cells (ECP-treated) has in the prevention of T1D in NOD mice. We discovered that five weekly injections of ECP-treated NOD spleen cells, beginning at 8 weeks of age, significantly delayed diabetes onset. Furthermore, cell dose studies demonstrated that low dose ECP-treated spleen cells (2 × 105 cells/injection/mouse) had similar protective effects as compared to high dose (5 × 106 cells/injection). In contrast to ECP-treated cells alone, ECP-treated cells combined with β cell antigens appeared to improve the protective effect as shown by the marked reduction in insulitis in the islets. Delivery of ECP-treated spleen cells or ECP-treated spleen cells plus β cell antigen increased Foxp3+ Tregs, and β cell antigen-specific T cell proliferation was significantly suppressed in vivo in these two groups. In addition, we found that ECP-treated cells did not induce global immunosuppression or autoimmunity against nuclear antigens. In conclusion, ECP-treated cells provide a safe and effective approach in T1D prevention, suggesting that clinical ECP has great potential for managing human T1D.
Journal: Clinical Immunology - Volume 135, Issue 3, June 2010, Pages 374–383