کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3258392 | 1207452 | 2008 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Clonal CD8+ TCR-Vβ expanded populations with effector memory phenotype in Churg Strauss Syndrome
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
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چکیده انگلیسی
Churg Strauss Syndrome (CSS) is a systemic vasculitis in which oligoclonal T cell expansions might be involved in the pathogenesis. Combined analysis of TCR-Vβ expression profile by flow cytometry and of TCR gene rearrangement by heteroduplex PCR was used to detect and characterize T cell expansions in 8 CSS patients, 10 asthmatics and 42 healthy subjects. In all CSS patients one or two Vβ families were expanded among CD8+ cells, with an effector memory phenotype apt to populate tissues and inflammatory sites. Heteroduplex PCR showed the presence of one or more clonal TCR rearrangements, which reveals monoclonal or oligoclonal T cells subpopulations. After purification with a Vβ specific monoclonal antibody, each CD8+/Vβ+ expanded family showed a single TCR rearrangement, clearly suggestive of monoclonality. All CD8+ expansions were detectable throughout the disease course. TCR-Vβ expanded or deleted populations were not observed in asthmatic patients. Clonal CD8+/Vβ+ T cell expansions might be useful as a disease marker.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinical Immunology - Volume 128, Issue 1, July 2008, Pages 94-102
Journal: Clinical Immunology - Volume 128, Issue 1, July 2008, Pages 94-102
نویسندگان
Giuseppe Guida, Antonella Vallario, Stefania Stella, Monica Boita, Paola Circosta, Sara Mariani, Giuseppina Prato, Enrico Heffler, Roberta Bergia, Antonino Sottile, Giovanni Rolla, Alessandro Cignetti,