کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3285587 | 1209233 | 2007 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Neutrophil Recruitment and Barrier Impairment in Celiac Disease: A Genomic Study
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کلمات کلیدی
RT-PCRqRT-PCRnatural killer - (سلول های) کشنده طبیعیMPO - DFOIfng - ifnginterleukin - اینترلوکینCeliac disease - بیماری سلیاکanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of variancemyeloperoxidase - میلوپراکسیداز quantitative reverse-transcription polymerase chain reaction - واکنش زنجیره ای پلیمراز کمی معکوس رونویسیInterferon gamma - گاما اینترفرون
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
بیماریهای گوارشی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Background & Aims: Celiac disease is an enteropathy featuring villous atrophy, crypt hyperplasia, and lymphocytosis. Tissue remodeling is driven by an inflammatory reaction to gluten in genetically susceptible individuals. The adaptive pathway is considered the major immune response but recent evidence has indicated the involvement of innate immunity as well. To assess the contribution of either immune response we performed global gene expression profiling of the regenerating mucosa. Methods: Microarray hybridizations were performed with biopsy samples from 13 untreated patients, 31 patients on a gluten-free diet in various stages of remission, and 21 controls. Additional data were generated using low-density array and conventional quantitative reverse-transcription polymerase chain reaction, and immunohistochemistry. Results: A total of 108 differentially expressed immune-related genes were identified (50 innate, 43 adaptive, 9 both innate/adaptive, and 6 immunoregulatory). Expression levels showed a gradual change as opposed to the discrete histological transitions. In addition to details provided on the adaptive and innate immune pathways used, we observed a chronic recruitment of activated neutrophils. Neutrophil involvement was unabated in otherwise completely normalized remission patients. Conclusions: We observed a contribution of both the innate and adaptive immune response in celiac disease pathogenesis. The discrepancy between the histological classification and the observed incremental change in immune-gene expression may have consequences for current diagnostic inclusion criteria. Enhanced neutrophil infiltration in both active and remission patients points to a genetic impairment of the intestinal barrier that may contribute to the cause rather than the consequence of celiac disease.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinical Gastroenterology and Hepatology - Volume 5, Issue 5, May 2007, Pages 574-581.e5
Journal: Clinical Gastroenterology and Hepatology - Volume 5, Issue 5, May 2007, Pages 574-581.e5
نویسندگان
Begoña Diosdado, Harm van Bakel, Eric Strengman, Lude Franke, Erica van Oort, Chris J. Mulder, Cisca Wijmenga, Martin C. Wapenaar,