کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3296161 1209865 2011 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Stromal Regulation of Human Gastric Dendritic Cells Restricts the Th1 Response to Helicobacter pylori
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی بیماری‌های گوارشی
پیش نمایش صفحه اول مقاله
Stromal Regulation of Human Gastric Dendritic Cells Restricts the Th1 Response to Helicobacter pylori
چکیده انگلیسی

Background & AimsMucosal dendritic cells (DCs) play a key role in initiating the T-helper (Th)1 response to Helicobacter pylori. To further elucidate the mucosal response to H pylori, we examined whether gastric stromal factors condition DCs to support tolerance to H pylori, analogous to intestinal stromal factor–driven macrophage tolerance to commensal bacteria.MethodsTo model mucosal DC development, we isolated and cultured cell-depleted human stroma/extracellular matrix from fresh gastric and intestinal mucosa to generate stroma-conditioned media. We then analyzed the capacity of stroma-conditioned media–treated monocyte-derived DCs and primary human gastric and intestinal DCs pulsed in vitro with H pylori to induce T-cell proliferation and interferon gamma secretion.ResultsStromal factors in gastric mucosa suppressed H pylori–stimulated DC activation and the ability of DCs to drive a Th1 proliferative and cytokine response to H pylori. The ability of gastric stromal factors to down-regulate DC function was similar to that of intestinal stromal factors and was independent of transforming growth factor β, prostaglandin E2, interleukin (IL)-10, and thymic stromal lymphopoietin. Stroma-conditioned media–induced reduction in DC-stimulated Th1 responses was associated with reduced DC release of IL-12.ConclusionsGastric stromal factors down-regulate DC responsiveness to H pylori, resulting in a dampened gastric Th1 response. We speculate that stroma-induced down-regulation of DC function contributes to the permissiveness of both gastric and intestinal mucosa to colonization by persistent residential microbes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gastroenterology - Volume 141, Issue 3, September 2011, Pages 929–938
نویسندگان
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