کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3328351 | 1212308 | 2013 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Masowe badania molekularne dla identyfikacji dawców ze sÅabÄ
ekspresjÄ
antygenu D
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
One of the basic rules of transfusion is to prevent alloimmunisation by highly immunogenic RhD antigen and not to transfuse RhD negative individuals with RhD positive blood. In some cases the expression of this antigen is so weak that it is undetectable in routine serological tests. The paper presents an algorithm for mass DNA testing with the purpose of identifying donors with low RhD expression. The screening is based on RHD gene detection (absent in RhD negative Caucasians) in DNA isolated from pools of 96 plasma samples from blood donors identified as RhD negative by the routine serological methods in Blood Transfusion Centers. Such procedure substantially reduces the costs. Plasma samples can be brought from the territory of the whole country, stored and accumulated for testing in one selected center. The cost would amount to approximately 25 PLN per donor. The test is performed once for each RhD negative individual. It is estimated that such persons will represent approximately 0.2% of RhD negative donors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Haematologica Polonica - Volume 44, Issue 3, JulyâSeptember 2013, Pages 260-264
Journal: Acta Haematologica Polonica - Volume 44, Issue 3, JulyâSeptember 2013, Pages 260-264
نویسندگان
Ewa Brojer, Katarzyna Guz, Agnieszka OrziÅska, Monika Pelc-KÅopotowska, BogumiÅa Michalewska,