کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3369625 1219044 2010 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Impact of extensive HIV-1 variability on molecular diagnosis in the Congo basin
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Impact of extensive HIV-1 variability on molecular diagnosis in the Congo basin
چکیده انگلیسی

BackgroundPrevious studies have shown a high HIV-1 genetic variability in the Republic of Congo. This can greatly influence the performance of molecular assays for HIV-1 diagnosis.ObjectivesTo define a reliable test for detection of HIV-1 DNA in this area.Study designWe compared a commercial nested PCR (C-PCR) assay and an in house nested PCR (H-PCR) assay for the detection of HIV-1 DNA in the first 30 seropositive pregnant women enrolled into the ongoing “Kento-Mwana” project, for the prevention of HIV mother-to-child transmission in the city of Pointe Noire, Republic of Congo, Africa. Sequencing and phylogenetic analysis of partial HIV-1 pol sequences were also performed.ResultsC-PCR detected HIV-1 DNA in only 15/30 samples from seropositive women (50.0%), as opposed to 29/30 (96.6%) by H-PCR (P < 0.0001). Phylogenetic analysis and bootscanning showed that only 10 sequences could be assigned to known clades (seven pure subtypes and three circulating recombinant forms), whereas the other 20 sequences were unique recombinant forms.ConclusionsThe great genetic variability of HIV-1 in this area strongly advises to for using molecular methods only after local validation to avoid false negative results.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Clinical Virology - Volume 47, Issue 4, April 2010, Pages 372–375
نویسندگان
, , , , , , , , , ,