کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3369956 | 1219060 | 2008 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Comparison of two highly automated DNA extraction systems for quantifying Epstein–Barr virus in whole blood Comparison of two highly automated DNA extraction systems for quantifying Epstein–Barr virus in whole blood](/preview/png/3369956.png)
BackgroundOptimal automated molecular methods are needed to monitor Epstein–Barr virus (EBV) infections in transplant recipients.ObjectivesTo compare the extraction of EBV DNA from whole blood using the COBAS Ampliprep™ and the MagNA Pure™ instruments (Roche) for quantifying EBV DNA by real-time PCR.Study designEBV DNA content was determined on clinical samples extracted by both systems.ResultsThe detection limit was 2.16 log10 copies/mL using the COBAS Ampliprep™ extraction system. Specificity was 100% and we saw no cross-contamination. Extraction was linear from 2.60 to 5.60 log10 copies/mL. The intra-assay variation was 1.91% for 3.60, 2% for 4.60 and 4.51% for 5.60 log10 copies/mL; inter-assay variation was 4.88%.Sixty-six samples were tested: 26 were positive and 28 were negative by both methods. One sample was MagNA Pure™ positive/COBAS Ampliprep™ negative (virus load 3.15 log10 copies/mL) and 10 samples were MagNA Pure™ negative/COBAS Ampliprep™ positive (virus loads from 1.59 to 3.51 log10 copies/mL) (P < 0.0001). Both methods gave similar quantitative results (average difference 0.07 log10 copies/mL) which were well correlated (r = 0.73, P < 0.001).ConclusionsThe COBAS Ampliprep™ extraction system is comparable to the MagNA Pure™ and offers a high reliability for extracting EBV DNA from whole blood.
Journal: Journal of Clinical Virology - Volume 43, Issue 3, November 2008, Pages 272–276