کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3370102 1219066 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Genetic diversity of noroviruses and sapoviruses in children with acute sporadic gastroenteritis in New Delhi, India
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Genetic diversity of noroviruses and sapoviruses in children with acute sporadic gastroenteritis in New Delhi, India
چکیده انگلیسی

BackgroundHuman caliciviruses (HuCVs) cause gastroenteritis throughout the world. Limited information is available on molecular epidemiology of caliciviruses from developing countries including India.ObjectivesStandardization and evaluation of a two-step multiplex RT-PCR assay for HuCVs and characterization of strains.Study designTwo hundred and twenty-six stool samples were collected from children with acute gastroenteritis (AGE) over a one and half year to study the prevalence and diversity of HuCVs in children with AGE in New Delhi, India. A multiplex two-step RT-PCR using 3 sets of external and 4 sets of internal primers from the RdRp gene was standardized for detection of NoVs and SaVs. Molecular characterization of some HuCV strains was done by sequencing followed by phylogenetic analysis.ResultsFifty-nine HuCVs strains were detected in 54 (24%) of the samples; 5 samples had mixed infections. Of these 59 HuCVs, 36 (61%) were norovirus (34 were GGII; 2 were GGI) and 23 (39%) were sapovirus (22 were GGI; 1 was GGII). Phylogenetic analysis of partial RdRp gene of 12 HuCV strains identified three genotypes (GGI/4, GGII/3 and a newly identified GIIb/Hilversum cluster) in NoVs and one genotype (GGI/1) in SaVs.ConclusionThis is one of the few reports from India on detection and characterization of HuCVs by multiplex RT-PCR assay. This assay can be a useful tool for epidemiological studies of HuCV infections.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Clinical Virology - Volume 43, Issue 1, September 2008, Pages 42–48
نویسندگان
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