کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3370650 1219084 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Detection and characterisation of human metapneumovirus from children with acute respiratory symptoms in north-west England, UK
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Detection and characterisation of human metapneumovirus from children with acute respiratory symptoms in north-west England, UK
چکیده انگلیسی

BackgroundHuman metapneumovirus (hMPV) causes a spectrum of respiratory disease ranging from trivial coryzal symptoms to fatal pneumonia, with a predilection for the very young, the immune suppressed and the frail elderly. Five distinct lineages of the virus genome have been described.ObjectivesTo develop and evaluate a sensitive, real-time PCR (RT-PCR) assay capable of detecting all lineages of hMPV, suitable for use in a diagnostic laboratory.Study designAn RT-PCR assay was developed using novel primers and dual-labelled minor-groove-binding (MGB) probes complementary to consensus sequences. The assay and two alternative assays were tested against external quality assurance (EQA) panels. 221 respiratory samples collected during 2003–2004 were screened using the new assay. hMPV positive samples were sequenced and phylogenetically analysed.ResultsThree genetic lineages of hMPV were detected during 2003–2004. Incidence was low (2.3%) compared to previous years. All five lineages had been present in the same community within the past 3 years.ConclusionsThe new assay correctly identified more EQA samples, including those at greatest dilution, than the alternative assays and detected all five lineages. Seasonal circulation of hMPV in paediatric patients with acute respiratory symptoms is dynamic with respect to incidence and viral genotype.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Clinical Virology - Volume 42, Issue 3, July 2008, Pages 273–279
نویسندگان
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