کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3370787 1219088 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Strain-specific antibody response to Lassa virus in the local population of west Africa
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Strain-specific antibody response to Lassa virus in the local population of west Africa
چکیده انگلیسی

BackgroundLassa virus (LAV) isolates obtained from Sierra Leone, Ivory Coast and Nigeria show a high degree of amino acid difference [Gunther S, Weisner B, Roth A, Grewing T, Asper M, Drosten C, et al. Lassa fever encephalopathy: Lassa virus in cerebrospinal fluid but not in serum. J Infect Dis 2001;184:345–9]. Therefore, three LAV strains from Sierra Leone, Ivory Coast and Nigeria were used as antigens to study the anti-LAV antibody response in 960 serum samples obtained from different regions of west Africa.Study designThe antibody response to LAV was studied both by a standard indirect immunofluorescence assay (IFA) and by a highly sensitive reverse ELISA [Emmerich P, Thome-Bolduan C, Drosten C, Gunther S, Ban E, Sawinsky I, et al. Reverse ELISA for IgG and IgM antibodies to detect Lassa virus infections in Africa. J Clin Virol 2006;37:227–81].ResultsIn 88 of the 960 subjects from west African countries (Guinea, Liberia, Ivory Coast, Ghana, Benin, and Nigeria) anti-Lassa antibodies were detected with both assays. Significant titer differences and clustering analysis revealed strain-specific antibodies in 64 of the 88 positive samples. Depending on the geographic origin of the samples, up to 32% of anti-LAV antibody positive samples would not have been detected, if only the IFA had been run with LAV prototype strain Josiah. In 20 patients with acute Lassa fever differences in antibody titer between the three LAV antigens were not observed.ConclusionsOur data suggest that antigens prepared of regional LAV strains should be applied when seroprevalence studies are conducted in various parts of west Africa.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Clinical Virology - Volume 42, Issue 1, May 2008, Pages 40–44
نویسندگان
, , ,