کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3371320 1219120 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rhinovirus/enterovirus RNA in tonsillar tissue of children with tonsillar disease
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Rhinovirus/enterovirus RNA in tonsillar tissue of children with tonsillar disease
چکیده انگلیسی

BackgroundHuman rhinoviruses (HRVs) together with the closely related human enteroviruses (HEVs) cause most of the acute respiratory illnesses throughout the year. HRVs have been detected in most parts of the respiratory tract but not in pharyngeal tonsils.ObjectivesWe aimed to find out whether HRVs were detectable in tonsillar tissue and if their presence correlated to the tonsillar disease.Study designThirty-three tonsillar samples collected in February–March 2003 from children with no acute respiratory symptoms were studied with HRV in situ hybridization (HRV-ISH). Ten tonsillar samples were further examined in a separate laboratory by two different reverse transcription polymerase chain reaction (RT-PCR) methods designed for detection of HRV/HEV RNA.ResultsTwenty of the 33 samples (62%) were positive by HRV-ISH. Five positive and five negative HRV-ISH samples were investigated by two different PCR methods. HRV/HEV RNA was detected in 9 of the 10 specimens by a hanging drop-nested PCR. One HRV-ISH negative sample was positive by a conventional non-nested PCR. One of the samples studied by all three methods, from a patient with recurrent tonsillitis, had no detectable HRV/HEV RNA. Positive result in HRV-ISH did not correlate significantly with underlying tonsillar disease, history of respiratory infections or bronchial asthma. Altogether HRV/HEV RNA was detected in 75% of the tonsils with no correlation to patients’ operation indication or history of respiratory diseases.ConclusionsIn February–March, HRV/HEV RNA was frequently found in tonsillar tissue in children irrespective of the tonsillar pathology. Whether detection of the RNA is a marker of chronic infection or is merely remnant of past infection is not known.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Clinical Virology - Volume 35, Issue 3, March 2006, Pages 292–297
نویسندگان
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