کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3393806 1592791 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of a loop-mediated isothermal amplification method for rapid mass-screening of sand flies for Leishmania infection
ترجمه فارسی عنوان
توسعه یک روش تقویت ایزوترمال متصل به حلقه برای غربالگری سریع مگس های شن و ماسه برای عفونت لیشمانیا
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
چکیده انگلیسی


• A colorimetric LAMP method of sand flies for Leishmania infection was established.
• The LAMP method could detect 0.01 parasites from crude sand fly.
• The method will be a useful tool for epidemiological studies of leishmaniasis.

Entomological monitoring of Leishmania infection in leishmaniasis endemic areas offers epidemiologic advantages for predicting the risk and expansion of the disease, as well as evaluation of the effectiveness of control programs. In this study, we developed a highly sensitive loop-mediated isothermal amplification (LAMP) method for the mass screening of sand flies for Leishmania infection based on the 18S rRNA gene. The LAMP technique could detect 0.01 parasites, which was more sensitive than classical PCR. The method was robust and could amplify the target DNA within 1 h from a crude sand fly template without DNA purification. Amplicon detection could be accomplished by the newly developed colorimetric malachite green (MG)—mediated naked eye visualization. Pre-addition of MG to the LAMP reaction solution did not inhibit amplification efficiency. The field applicability of the colorimetric MG-based LAMP assay was demonstrated with 397 field-caught samples from the endemic areas of Ecuador and eight positive sand flies were detected. The robustness, superior sensitivity, and ability to produce better visual discriminatory reaction products than existing LAMP fluorescence and turbidity assays indicated the field potential usefulness of this new method for surveillance and epidemiological studies of leishmaniasis in developing countries.

Loop-mediated isothermal amplification (LAMP) method for the mass screening of sand flies for Leishmania infection.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Tropica - Volume 132, April 2014, Pages 1–6
نویسندگان
, , , , , , , , , ,